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After 24 h from seeding, different concentrations of MGL in the range 0.00050.5 mg/ml in a sterile PBS solution containing 100 M PLP, pH 7.4, were added to DMEM medium supplemented with 5% FBS and 0.5 mM PLP in PBS, and co-incubated for 24, 48, and 72 h

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Deferoxamine (DFO) is an FDA-approved iron chelator that binds non-transferrin-bound free iron [110,111,112]

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